As soon as a living organism dies, it stops taking in new carbon.The ratio of carbon-12 to carbon-14 at the moment of death is the same as every other living thing, but the carbon-14 decays and is not replaced.This is calculated through careful measurement of the residual activity (per gram C) remaining in a sample whose age is Unknown, compared with the activity present in Modern and Background samples. Thus 1950, is year 0 BP by convention in radiocarbon dating and is deemed to be the 'present'.You can get an idea of the relationship between C14 and age at the Carbon Dating calculator page. 1950 was chosen for no particular reason other than to honour the publication of the first radiocarbon dates calculated in December 1949 (Taylor, 19).Later inter-laboratory measurements put the ratio at 1.5081 (Currie and Polach, 1980).According to Stuiver and Polach (1977), all laboratories should report their results either directly related to NBS Oxalic acid or indirectly using a sub-standard which is related to it.The Oxalic acid standard was made from a crop of 1955 sugar beet. The isotopic ratio of HOx I is -19.3 per mille with respect to (wrt) the PBD standard belemnite (Mann, 1983). T designation SRM 4990 C) was made from a crop of 1977 French beet molasses.
Amongst accelerator laboratories there has been mooted the theoretical possibility of extended range dating to 75 000 yr , at present this seems difficult to attain because of the problems in accurately differentiating between ions that mimic the mass and charge characteristics of the C14 atom.Another standard, Oxalic Acid II was prepared when stocks of HOx 1 began to dwindle. The ratio of the activity of Oxalic acid II to 1 is 1.29330.001 (the weighted mean) (Mann, 1983). There are other secondary radiocarbon standards, the most common is ANU (Australian National University) sucrose.The ratio of the activity of sucrose with 0.95 Ox was first measured by Polach at 1.50070.0052 (Polach, 1976b:122).d14C represents the per mille depletion in sample carbon 14 prior to isotopic fractionation correction and is measured by: D14C represents the 'normalized' value of d14C.'Normalized' means that the activity is scaled in relation to fractionation of the sample, or its delta C13 value.